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Dr. Jeremy A. Bruenn

Professor
Cooke 661
phone: (716) 645-4967
email: cambruen@buffalo.edu
Further research info

Research Summary

Molecular virology: This lab has worked on the molecular virology of small dsRNA viruses for over 25 years. Currently we are exploring the nature of the RNA-protein interaction that results in packaging of the viral plus strand. We have explored the RNA recognition site via PCR amplification techniques and defined the site as a small (20 base) region with a specific secondary structure. Similarly, the region of the large protein (over 1500 amino acids) that recognizes this sequence has been delimited to about 150 amino acids. We are now in the process of mutating each of these residues one at a time to map the contact residues.

Selected Publications

• Tu, C.-L., Tzeng, T.-H., and Bruenn, J.A. (1992). Ribosomal movement impeded at a pseudoknot required for frameshifting. Proc. Natl. Acad. Sci. USA 89, 8636-8640.
• Yao, W., Adelman, K., and Bruenn, J.A. (1997). In vitro selection of packaging sites in a double-stranded RNA virus. J. Virol. 71, 2157-2162.
• Routhier, E. and Bruenn, J.A. (1998). Functions of conserved motifs in the RNA-dependent RNA polymerase of a yeast double-stranded RNA virus. J. Virol. 72, 4427-4429.
• Lopinski, John D., Dinman, Jonathan D., and Bruenn, Jeremy A. (2000). Kinetics of ribosomal pausing during programmed -1 translational frameshifting. Molec. Cell. Biol. 20, 1095-1103.
• Bruenn, J. (2003). A structural and primary sequence comparison of the viral RNA dependent RNA polymerases. Nucleic Acids Res. 31, 1821-1829.