Research Summary
Our lab is interested in understanding the cell biological mechanisms of axon outgrowth and pathfinding, both during nervous system development and in the context of peripheral neuropathy. Peripheral neuropathy is an extremely common condition and can occur as a primary neurologic syndrome (e.g. due to injury or an inherited peripheral neuropathy) or as a secondary consequence of another disease or syndrome (e.g. due to diabetes). It is also a common side effect of treatment with a number of pharmaceutical compounds, particularly those with chemotherapeutic applications. We have devised a rapid and efficient assay system to examine the behavioral and cellular consequences of treatment with neuropathy-inducing compounds in the zebrafish and are current pursuing three primary goals:
1. Understanding the cell-biological events that lead to peripheral neuropathy.
2. Understanding how genetic background determines whether or not an individual is likely to suffer from harmful side effects of non-genetic factors, such as a drug.
3. Screening compounds for their potential to cause peripheral neuropathy as a side effect.
Selected Publications
Research:
- Hutson, LD, Campbell, DS, and Chien, C-B. “Analyzing axon guidance in the zebrafish retinotectal system” in Essential Zebrafish Methods: Cell and Developmental Biology. Ed. Monte Westerfield, Leonard Zon, H. Dietrich III. Academic Press. 2009.
- Marvin, MJ, O’Rourke, D*, Kurihara T*, Juliano, CJ*, Harrison, KL*, and Hutson, LD (2008) Developmental expression patterns of the zebrafish small heat shock proteins. Dev Dyn. 237(2):454-63.
- Elicker, KS* and Hutson, LD (2007) Genome-wide analysis and expression profiling of the zebrafish small heat shock proteins. Gene, 403(1-2):60-9.
- Barresi, MJ, Hutson, LD, Chien, CB, and Karlstrom, RO. (2005) Hedgehog regulated Slit expression determines commissure and glial cell position in the zebrafish forebrain. Development. 132(16):3643-56
- Miyasaka, N, Sato, Y, Yeo, SY, Hutson, LD, Chien, CB, Okamoto, H, and Yoshihara, Y (2005) Robo2 is required for establishment of a precise glomerular map in the zebrafish olfactory system. Development. 132(6):1283-93.
- Hutson, LD, Campbell, DS, and Chien, C-B (2004) Analyzing axon guidance in the zebrafish retinotectal system. Methods Cell Biol. 76:13-35. (Invited review)
- Hutson LD, Jurynec MJ, Yeo SY, Okamoto H, Chien C-B (2003) Two divergent slit1 genes in zebrafish. Dev Dyn. 228(3):358-69.
- Hutson, LD and Chien, C-B (2002) Wiring the zebrafish: axon guidance and synaptogenesis. Curr Opin Neurobiol. 12(1):87-92. (Invited review)
- Hutson, LD and Chien, C-B (2002) Pathfinding and error correction by retinal axons: the role of astray/robo2. Neuron. 33(2):205-17.
- Hutson, LD and Bothwell, M (2001) Expression and function of Xenopus laevis p75(NTR) suggest evolution of developmental regulatory mechanisms. J Neurobiol. 49(2):79-98.
Education:
- Hutson LD and Liang JO (2009) Making an Impact: Zebrafish in Education. Zebrafish. 6(2):119-120. (editorial)
- Ekker SC, Hutson LD, Fields M (2009) The Art and Science of Zebrafish in the Classroom—An Interview with Melanie Fields. Zebrafish. 6(2): 121-126.
- McKeown KA, Downes GB, Hutson LD (2009) Modular Laboratory Exercises to Analyze the Development of Zebrafish Motor Behavior. Zebrafish. 6(2): 179-185.
- Barresi MJ, Farber SA, Hutson LD, Hyde DR, Liang JO, Morris JK. (2008) Zebrafish in the Classroom. Zebrafish. 5(3):205-208.
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