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cullenDr. Paul J. Cullen

Office: Cooke 341
Office Hours: Friday 2:00 – 5:00 pm
Tel: 716-645-4923
Fax: 716-645-2975

Ph.D. Washington University, Saint Louis
Postdoctoral Research at University of Oregon

Cullen Lab Website

Research Summary

Our lab is interested in signal transduction pathway regulation in the model organism budding yeast. We investigate cell-surface receptors that activate the GTPase Cdc42 and connect to MAP kinase regulatory proteins. We use genetic, biochemical, and genome-wide approaches to investigate questions related to pathway activation, specification and attenuation.

Dr. Cullen’s CV

Postdoctoral Opportunities in the Cullen Lab

Selected Publications

  • Kumar, R., Breindel, C., Saraswat, D., Cullen, P.J. and M. Edgerton (2017) Candida albicans Sap6 Amyloid Regions Function in Cellular Aggregation and Zinc Binding, and Contribute to Zinc Acquisition. Scientific Reports. 7:1p2908.
  • Gonzalez, B. Mas, A., Beltran, G., Cullen, P.J. and Torija, M.J. (2017) Retrograde Mitochondria Pathway Regulates Ethanol-Inducible Filamentous Growth in Yeast.  Frontiers in Physiology. 8:148.  doi: 10.3389/fphys.2017.00148.
  • Woolford, C.A., Lagree, K., Xu, W., Aleynikova, T., Adhikari, H., Sanchez, H., Cullen, P.J., Lanni, F., Andes, D.R., and AP Mitchell (2016) Biofilm formation in the absence of Candida albicans biofilm transcriptional activators. PLoS Genetics. DOI:10.1371/journal.pgen.1006487.
  • Basu, S., Vadaie, N., Pradhakbar, A. Li, B., Pitoniak, A., Adhikari, H., Chavel, C., and Cullen, P.J. (2016) Spatial Landmarks Regulate a Cdc42p-Dependent MAPK Pathway to Control Differentiation and the Response to Positional Compromise. PNAS. 113(14):E2019-28.
  • Saraswat, D. Kumar, R., Pande, T. Edgerton M. and Cullen, P.J. (2016) Signaling Mucin Msb2 Regulates Adaptation to Thermal Stress in Candida albicans. Mol. Microbiology100(3):425-41.
  • Cullen, P.J. and Edgerton M. (2016) Unmasking Fungal Pathogens by Studying MAPK-Dependent Cell Wall Regulation in Candida albicans. Virulence. Apr 18:1-4.
  • Cullen, P.J. (2015) The Plate-Washing Assay: A Simple Test for Filamentous Growth in Budding Yeast. Cold Spring Harbor Laboratory Protocols. (2):168-71.
  • Cullen, P.J. (2015) Evaluating Yeast Filamentous Growth at the Single-Cell Level. Cold Spring Harbor Laboratory Protocols. (3): 272-5.
  • Cullen, P.J. (2015) Evaluating the Activity of the Filamentous Growth Mitogen Activated Protein Kinase Pathway in Yeast. Cold Spring Harbor Laboratory Protocols. (3):276-83.
  • Cullen, P.J. (2015) Biofilm/Mat Assays for Budding Yeast. Cold Spring Harbor Laboratory Protocols. (2): 172-5.
  • Cullen, P.J. (2015) Investigating Filamentous Growth and Biofilm/Mat Formation in Budding Yeast. Cold Spring Harbor Laboratory Protocols. (3): 235-8.
  • Adhikari, H., Caccamise, L.M., Pande, T., and Cullen, P.J (2015) Comparative Analysis of Transmembrane Regulators of the Filamentous Growth MAPK Pathway Uncovers Functional and Regulatory Differences. Eukaryotic Cell. 14(9):868-883.
  • Adhikari, H. Vadaie, N., Chow, J., Caccamise, L.M., Chavel, C.A., Li, B., Bowitch, A. Stefan, C.J., and Cullen, P.J. (2015) Role of the Unfolded Protein Response in Regulating the Mucin-Dependent Filamentous-Growth Mitogen Activated Protein Kinase Pathway. Molecular and Cellular Biology. 35(8):1414-32.
  • Adhikari, H. and Cullen, P.J. (2015) Role of Phosphatidylinositol Phosphate Signaling In the Regulation of the Filamentous Growth MAPK Pathway. Eukaryotic Cell. 14(4): 427-40.
  • Li, R. Puri, S. Tati, S. Cullen, P.J. and Edgerton, M. (2015) Candida albicans Cek1 MAPK signaling enhances fungicidal activity of salivary Histatin. Antimicrobial Agents and Chemotherapy. 59(6):3460-8.
  • Pitoniak A., Chavel C.A., Chow J., Smith J., Camara D., Karunanithi S., Li B., Wolfe K., Cullen P.J. (2015) Cdc42p-Interacting Protein Bem4p Regulates the Filamentous Growth MAP Kinase Pathway. Mol. Cell. Biol. Nov 10. pii: MCB.00850-14.
  • Chavel C.A., Caccamise L.M., Li B., Cullen P.J. (2014) Global regulation of a differentiation MAPK pathway in yeast. Genetics. V198(3):1309-28.
  • Adhikari, H. and Cullen P.J. (2014) Metabolic respiration induces AMPK- and Ire1p-dependent activation of the p38-Type HOG MAPK pathway. PLoS Genetics. V10(10):e1004734. doi: 10.1371/journal.pgen.1004734.
  • Puri, S., Kumar, R., Chadha, S., Tati S., Conti, H.R., Hube, B., Cullen, P.J, Edgerton M. (2012) Secreted Aspartic Protease Cleavage of Candida albicans Msb2 Activates Cek1 MAPK Signaling Affecting Biofilm Formation and Oropharyngeal Candidiasis. PLoS ONE. 7(11):e46020.
  • Karunanithi S., and Cullen P.J. (2012) The Filamentous Growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 Transcriptional Repressors in Saccharomyces cerevisiae. Genetics. 192(3):869-87.
  • Meem M.H. and Cullen P.J. (2012) The impact of protein glycosylation on Flo11-dependent adherence in Saccharomyces cerevisiae. FEMS Yeast Research. 12(7):809-18.
  • Karunanithi S, Joshi J, Chavel C, Birkaya B, Grell L, and P.J. Cullen (2012)Regulation of Mat Responses by a Differentiation MAPK Pathway in Saccharomyces cerevisiae. PLoS ONE. 7(4): e32294
  • Cullen, P.J. and G.F. Sprague (2012) The regulation of filamentous growth in yeast. Genetics. V190:23-49.
  • Cullen, P.J. (2011) Post-translational Regulation of Signaling Mucins. Current Opinion of Structural Biology. V21 p590-596.
  • Karunanithi, S., Vadaie, N., Chavel, C., Birkaya, B., Joshi, J., Grell, L, and P.J. Cullen (2010) Shedding of the Mucin-like Flocculin Flo11p Reveals a New Aspect of Fungal Adhesion Regulation. Current Biology. 20:1-7.
  • Chavel, C.A., Dionne, H.S., Birkaya, B. Joshi, J., and P.J. Cullen (2010) New Regulators of a Differentiation MAPK Pathway. PLoS Genetics. 19; 6(3):e1000883
  • Pitoniak, A., Birkaya, B., Dionne H.M., Vadaie, N., and P. J. Cullen (2009) The Signaling Mucins Msb2 and Hkr1 Differentially Regulate the Filamentation MAPK Pathway and Contribute to a Multimodal Response. Molecular Biology of the Cell. V20 p3101-3114.
  • Birkaya, B. Maddi, A., Joshi, J., Free, S.J., and P.J. Cullen (2009) The Role of the Cell Wall Integrity Pathway and Cdc42-Dependent MAPK Pathway in Cell Wall Remodeling During Filamentous Growth. Eukaryotic Cell. V8 p1118-1133.
  • Abdullah, U. and P.J. Cullen (2009) The tRNA Modification Complex Elongator Regulates the Cdc42-Dependent MAPK Pathway that Controls Filamentous Growth In Yeast. Eukaryotic Cell. V8 p1362-1372.
  • Vadaie, N., Dionne, H., Nickerson, S.R., Akajagbor, D.S., Krysan, D.J., and Cullen, P.J. (2008) Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast. J. Cell Biology. 181: 1073-1081.
  • Cullen, P.J. (2007) Signaling Mucins: The New Kids on the MAPK Block. Critical Reviews in Eukaryotic Gene Expression. 
  • P.J. Cullen, Xu-Friedman, R., Delrow, J., and G. F. Sprague, Jr. (2006).Genome-wide analysis of a protein glycosylation deficiency. FEMS Yeast Research.
  • Devit, M.J., Cullen, P.J. Branson, M., Sprague, G.F., and Fields, S. (2005).A novel genetic screening method based on artificially forced protein interactions. Genome Research. 15 (4):560-565.
  • Cullen, P.J., Sabbagh Jr., W., Graham, E., Irick, M. van Olden, E.K., Neal, C., Delrow, J., Bardwell, L., and George F. Sprague, Jr. (2004). A signaling mucin at the head of the Cdc42- and MAPK-filamentous growth pathway in yeast.Genes & Development. 18:1695-1708.
  • Smith, G.R., Given, S.A., Cullen, P.J., and Sprague, G.F. (2002)Identification and Characterization of GTPase Activating Proteins (GAPs) for Cdc42. Eukaryotic Cell. V1 p469-480. 
  • Cullen, P.J., and Sprague, G.F., Jr. (2002) The Glc7p-interacting protein Bud14p attenuates pheromone response, filamentous growth, and polarized growth in Saccharomyces cerevisiaeEukaryotic Cell. V1:884-894.
  • Cullen, P.J., and Sprague, G.F., Jr. (2002)The roles of bud-site-selection proteins in haploid invasive growth in yeast. Mol. Biol. Cell. 13:2990-3004.
  • Cullen, P.J. and Sprague, G.F. (2000) Glucose depletion causes haploid invasive growth in yeast. PNAS. 97: 13619-13624.*See Commentary on this article: Madhani, H.D. (2000) Interplay of intrinsic and extrinsic signals in yeast differentiation. PNAS. 97: 13461-13463.
  • Cullen, P.J., Schultz, J., Horecka, J., Stevenson, B., and Jigami, Y., and Sprague, G.F. (2000) Defects in protein glycosylation cause SHO1-dependent activation of a STE12 signaling pathway in yeast. Genetics. 155: 1005-1018.